Mice (mus Musculus) Genome Responses to Methotrexate (mtx) and Some Plant Extracts

Mice genome responses to MTX (anticancer drug) and some plant (Curcuma, Ginger, Green Tee and Pomegranate) extracts were studied. Mice were intraperitoneally injected with 0.75 mg/kg chronic dose of MTX for two weeks. Mortality percentages, bone marrow cell divisions, morphological and biochemical characterization of treated mice groups were estimated. The mortality percentage was 40 % in the positive control while no mortality was observed in both (MTX, Curcumin) and (MTX, Pomegranate) treatments. Some treated mice had ulceration and hair loss on the ears skin. No morphological changes were observed on the negative control group. At cytogenetic level, no bone marrow cell divisions were detected in treated mice. Two Isozyme systems (Esterase and Super oxide dismutase) and Protein electrophoresis were assayed to detect biochemical genetic markers for all mice groups. These analyses reflect mice genome responses under experimental conditions. [Mansour, A.A. M.A. Salam, and Y. M. Saad. Mice (Mus musculus) genome responses to methotrexate (MTX) and some plant extracts. Life Sci J 2012;9(4):4881-4886] (ISSN:1097-8135). http://www.lifesciencesite.com.733